Silica-coated Gd(DOTA)-loaded protein nanoparticles enable magnetic resonance imaging of macrophages J Mater Chem B. 2015 Oct 1;3(38):7503-7510. doi: 10.1039/C5TB01014D. Epub 2015 Jul 22. Authors Michael A Bruckman 1 ...

In one prominent example, Langhammer and co-workers employed the INPS strategy to characterize protein adsorption onto silica-coated gold nanodisks within the context of studying protein corona formation [37,38]. In addition to coating the nanoplasmonic transducer with a conformal dielectric layer, other materials can also be deposited on the ...

This invention defines gated silica nanoparticles into which chemical cargo materials or substances are embedded and of which surfaces are coated with biologically active gating molecules and apparatus employing such silica nanoparticles. The invention and the apparatus employing the invention have the potential to be used in diverse fields such as health, food …

The SCDDS were prepared by bHb immobilisation on mesoporous silica followed by myristic acid (MA) coating at 43 °C and 100 bar in scCO 2. bHb-immobilised silica particles were also coated via solvent evaporation (SE) to compare the protein release with scCO 2 processed formulations. In both cases, MA coating provided required enteric ...

Fluorescence-based DNA hybridization and protein binding assays can be improved using silica-coated gold nanoparticles as robust labels that do not degrade, quench, or photobleach. 17 This nanoparticle platform can also be employed to detect extremely low concentrations of proteins, bacteria, pesticides, and small molecules such as mercury.

The silica coating significantly altered the plasma protein corona composition and enhanced retention of 141 Ce in other organs except the liver. Conclusion We conclude that silica coating of nanoceria alters the biodistribution of cerium likely due to modifications in protein corona formation after IT and IV administration. Background

The suspension of silica coated magnetic beads are yellowish-brown. The structure of embedding type magnetic SiO2 microspheres is that Fe3O4 magnetic nanoparticles embedded in the monodisperse silica microspheres. This type of magnetic microspheres is superficially functionalized with rich silanol groups. The dispersant is phosphate buffer.

Stability of Chlorhydrol-coated silica colloids in so- lutions of increasing protein concentration. Derivatized silica beads were added (final concentration, 6%) to buffered (140 mM sorbitol, 20 mM MES, pH 6.5) solutions of increasing concentrations of bovine serum albumin. After a short incubation (10 min), the

Silica is usually present in the form of films, colloidal suspensions, and powders of particles and fibers. The sodium silicate and Ludox® are commercial examples of colloidal suspensions, which have between 30 and 50 wt% SiO 2, nonporous nanoparticles (above 25 nm), and Na + or NH 4 + counterions in a basic aqueous medium. The charge of the deprotonated silanol groups in this …

Our silica coated gold nanoparticles are highly stable and suitable for applications including: Sensing/Detection 1, Biological Targeting 2, and enhanced photoacoustics upon aggregation 3.Silica coating has also been shown to enhance gold uptake in cells 4.

The silica gel material is coated by a phospholipid bilayer containing at least two lipids, at least one having an electric charge. Proteins are separated using this column packing material without denaturing because of the lipid coating. The protein molecule binds to the lipid bilayer coated silica gel and is then eluted by raising the ...

The expression profile of the exosomal protein of the silica-exposed macrophages changed. ... Inning am Ammersee, Germany). For morphological analysis, 10 μL of exosomes were dropped onto a Formvar‑carbon coated electron microscope slide for 1 min, absorbed the floating liquid, and then 10 μL 1% uranyl acetate was added for 1 min, removed ...

Silica-coated nanoparticles are widely used in biomedical applications such as theranostics, imaging, and drug delivery. While silica-coated nanoparticles are biocompatible, experimental evidence shows that they can trigger innate immune reactions, and a broader understanding of what types of reactions are caused and how to mitigate them is needed.

In this study we compare surfactant interactions with protein-coated silica surfaces differing in the degree of curvature (macroscopically flat and colloidal nanometric spheres). The interaction ...

NCI Thesaurus (NCIt) Silica is another name for the chemical compound composed of silicon and oxygen with the chemical formula SiO2, or silicon dioxide. There are many forms of silica. All silica forms are identical in chemical composition, but have different atom arrangements. Silica compounds can be divided into two groups, crystalline (or c ...

4.2.2. Ring-Opening Polymerization According to the article published by Cruz et al., a mesoporous, silica-coated Fe core-shell nanoparticle was synthesized via a surfactant template sol-gel method utilizing magnetic nanoparticles (MNPs) stabilized with nucleation seeds exhibiting superparamagnetic behavior [119].

These characteristics of silica-coated beads have found utility in cell-free protein synthesis, which was verified by the successful synthesis of EGFP in the beads including DNAs and protein synthesis machinery.

The loaded TMV particles were then coated with silica maintaining high relaxivities. Interestingly, we found that when Gd(DOTA) was loaded into the interior channel of TMV and the exterior was coated with silica, the T 1 relaxivities increased by three-fold from 10.9 mm −1 s −1 to 29.7 mm −1 s −1 at 60 MHz compared to uncoated Gd-loaded ...

For low to intermediate molecular weight proteins, Absolute Mag™ C8 Silica-coated Magnetic Particles are preferred. Absolute Mag™ C4 Silica-coated Magnetic Particles are most suitable for larger molecular weight proteins and peptides. In many cases, however the three types of beads can be used interchangeably.

DOI: 10.1039/B925137E Corpus ID: 24463448. Fabrication of mesoporous silica-coated CNTs and application in size-selective protein separation @article{Zhang2010FabricationOM, title={Fabrication of mesoporous silica-coated CNTs and application in size-selective protein separation}, author={Mingkang Zhang and Yipan Wu and Xizeng Feng and Xiwen He and …

Protein adsorption to silica surfaces is a notorious problem in analytical separations. Evanescent-wave cavity ring-down spectroscopy (EW-CRDS) and capillary electrophoresis (CE) were employed to investigate the capability of positively charged polymer coatings to minimize the adsorption of basic proteins. Adsorption of cytochrome c (cyt c) to silica coated …

Therefore, we conclude that the silica-coated protein-based contrast agent may provide an interesting candidate material for further investigation for in vivo delineation of disease through macrophage imaging. Introduction. Molecular imaging facilitates the early detection of disease, allows risk stratification, disease monitoring, longitudinal ...

184 S.Y. Lim et al. / Journal of Biotechnology 143 (2009) 183–189 Fig. 1. A scheme for the silica-coated alginate beads encapsulating the in vitro transcriptional and translational machinery for in vitro protein synthesis. EGFP was produced in silica-coated beads during their immersion in a feeding solution which contained resource materials.

NPs containing fluorescent proteins (i.e., mCherry and green fluorescent protein) and naturally occurring autofluorescent substances reportedly have non-specific adsorption potential [30,31,32,33], while non-specific adsorption of fluorescent dyes has not yet been reported for silica or cobalt ferrite.

Analyses of protein corona on bare and silica-coated gold nanorods against four mammalian cells Minakshi Das,1 Dong Kee Yi,2 Seong Soo A An1 1Department of Bionanotechnology, Gachon University, Seongnam, Republic of Korea; 2Department of Chemistry, Myongji University, Yongin, Republic of Korea Abstract: The purpose of this study was to investigate the …

Affinity Ligand Coated MicrospheresAffinity binding ligands can aid in the rapid development or streamlined production of microsphere reagents. They also provide the opportunity for optimal orientation of the ligand, as with end-point immobilization of biotinylated oligonucleotides to streptavidin-coated microspheres, or the Fc-specific immobilization of antibodies via Protein

Sandblasted acid-etched titanium (SAE-Ti) and a silica hybrid sol-gel coating (35M35G30T) applied onto the Ti substrate were examined. The results of in vitro and in vivo tests and the analysis of the protein layer adsorbed to each surface were compared and discussed.

SeraSil-Mag™ silica coated superparamagnetic beads for nucleic acid isolation deliver high purity DNA extraction for highly sensitive applications where sample is scarce. These beads provide an optimal surface for nucleic acid binding with high performance and low background.

Preparation and characterization of whey protein isolate films reinforced with porous silica coated titania nanoparticles Abstract Whey protein isolate (WPI) films embedded with TiO2@@SiO2 (porous silica (SiO2) coated titania (TiO2)) nanoparticles for improved mechanical properties were prepared by solution casting. A WPI solution

Therefore, we conclude that the silica-coated protein-based contrast agent may provide an interesting candidate material for further investigation for in vivo delineation of disease through macrophage imaging. Go to: Introduction. Molecular imaging facilitates the early detection of disease, allows risk stratification, disease monitoring ...

Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic …

The potential toxicity of nanoparticles, particularly to neurons, is a major concern. In this study, we assessed the cytotoxicity of silica …

A comparison of zeta potential (ζ) versus pH isotherms between the protein-coated SNP and native protein reveals deviation in ζ that can be attributed to perturbation in the protein structure. Analysis of the ζ versus pH isotherms for CP–protein–silica nanocomposites and the native CP indicates that CP deposition on SNPs occurs in a ...

silica shell thickness of approximately 10 and 30 nm, the procedures were virtually the same except that the concentrations of the QD chloroform solution were 5.5 and 1.1 lM, respectively. Pegylation of Silica Coated QDs Mesoporous silica coated nanoparticles were first functionalized with primary amines. During the silica

Silica-coated alginate beads successfully encapsulate transcriptional and translational machinery for the prolonged cell-free synthesis of functional protein. The silica coating, identified by optical microscope and energy-dispersive spectroscopy, made the beads more resistant-than bare or …

Silica particles enable oral protein delivery in mice. ... Furthermore, loaded capsules were coated with Eudragit L100-55, a pH-responsive polymer that remains intact at …